Stephen Mayfield was born about 1730 in Virginia Colony, and died after 1786. There is no credible evidence concerning the existence of a wife or children for this man. Accordingly, it is not known if Stephen was ever married.

This Stephen Mayfield is probably the same person who appears (along with a certain Robert Mayfield - probably his brother) on the 1766 tax list for Bute County, North Carolina. If Stephen is indeed a brother of Robert Mayfield (d. 1816 in Chester County SC), then he is probably a son of the Abraham Mayfield who died testate in Granville County, North Carolina in 1778.

Subsequent to the year 1771, Stephen apparently removed to the Browns Creek region of Ninety-Six District, South Carolina - probably at the same time as his brother Robert Mayfield. Stephen is mentioned on the 1774 land plat of a certain David George as having land adjacent to George's along Browns Creek. John Mayfield the Tory (probably a nephew of Stephen) also lived nearby.

During the Revolution, Stephen supported the Loyalist Cause and ultimately left the state, probably some time in late 1779. Unlike his kinsman, John Mayfield the Tory, he apparently refused to take the oath of allegiance to the Whig government of South Carolina and chose to depart the state. As a result, he was proscribed by the State of South Carolina and his name appears on the Proclamation List issued by South Carolina in December 1779.

The next mention of Stephen Mayfield in any official records is from the muster roll of the South Carolina Royalist Regiment, then stationed in Savannah, Georgia, The regimental muster roll for 1 December 1779 contains the name of Stephen Mayfield as a Lieutenant serving in the company of Captain John Murphy. Steven's date of commission is given as 13 November 1779. At this time the regiment was commanded by Joseph Robinson, the same man who, as a Loyalist militia captain, was active during the events leading up to the arrest of Colonel Thomas Fletchall in December 1775. Robinson escaped capture and fled to Florida where he ultimately raised a regiment in support of the Loyalist Cause.

Towards the end of the Revolution, Stephen Mayfield seems to have taken up residency in the British Colony of East Florida. For a period of time he ran a tavern in the northern part of the colony and was a consort of the infamous Tories Daniel McGirtt and Bloody Bill Cunningham. When the Treaty of Paris was signed in April 1783, East Florida was ceded to Spain and Stephen was ultimately forced to leave. The last mention of this Stephen Mayfield in the official records was in a letter dated 07 January 1786, written by a Spanish official. Stephen Mayfield had been expelled from Florida by the Spanish and was, at that time, in Providence in the Bahamas.